Analysis Note

ControlPositive = cerebral cortex. Negative = liver or kidney.

Application

Research CategoryNeuroscience

Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.

Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.

Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryNeuronal & Glial MarkersNeurofilament & Neuron Metabolism

Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified bovine brain MAP2

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.

Format: Purified

Specificity

Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

Recognizes a doublet of ~280 kDa and a doublet of ~70kDa